Dev113001 4231..4242
نویسندگان
چکیده
Mouse embryonic stem cells (mESCs) are clonal populations derived from preimplantation mouse embryos that can be propagated in vitro and, when placed into blastocysts, contribute to all tissues of the embryo and integrate into the normal morphogenetic processes, i.e. they are pluripotent. However, although they can be steered to differentiate in vitro into all cell types of the organism, they cannot organise themselves into structures that resemble embryos. When aggregated into embryoid bodies they develop disorganised masses of different cell types with little spatial coherence. An exception to this rule is the emergence of retinas and anterior cortex-like structures under minimal culture conditions. These structures emerge from the cultures without any axial organisation. Here, we report that small aggregates of mESCs, of about 300 cells, self-organise into polarised structures that exhibit collective behaviours reminiscent of those that cells exhibit in early mouse embryos, including symmetry breaking, axial organisation, germ layer specification and cell behaviour, as well as axis elongation. The responses are signal specific and uncouple processes that in the embryo are tightly associated, such as specification of the anteroposterior axis and anterior neural development, or endoderm specification and axial elongation. We discuss the meaning and implications of these observations and the potential uses of these structures which, because of their behaviour, we suggest to call ‘gastruloids’.
منابع مشابه
outputs The enumeration of permutations avoiding 2143 and 4231
We enumerate the pattern class Av(2143, 4231) and completely describe its permutations. The main tools are simple permutations and monotone grid classes.
متن کامل’ s repository of research publications and other research outputs The enumeration of permutations avoiding 2143 and 4231
We enumerate the pattern class Av(2143, 4231) and completely describe its permutations. The main tools are simple permutations and monotone grid classes.
متن کاملThe enumeration of permutations avoiding 2143 and 4231
We enumerate the pattern class Av(2143, 4231) and completely describe its permutations. The main tools are simple permutations and monotone grid classes.
متن کاملOn the Dual Canonical and Kazhdan-lusztig Bases and 3412, 4231-avoiding Permutations
Using Du’s characterization of the dual canonical basis of the coordinate ring O(GL(n,C)), we express all elements of this basis in terms of immanants. We then give a new factorization of permutations w avoiding the patterns 3412 and 4231, which in turn yields a factorization of the corresponding Kazhdan-Lusztig basis elements C w(q) of the Hecke algebra Hn(q). Using the immanant and factorizat...
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